The Duke of Flies
Boss The Duke of Flies.png
Found In
The Basement
The Cellar
The Catacombs (Double Trouble)
Boss Challenge Room
Challenge Room
Normal Enemy (Later Floors)
Dropped Items
Boss Pool
Unlocked By

The Duke of Flies is a boss that appears in The Basement. Compared to most other bosses, the Duke has little health and cannot even attack on his own. He makes up for this by summoning attack flies for both offensive and defensive purposes. The Duke of Flies was a boss in the original Binding of Isaac.

Behavior[edit | edit source]

The Duke of Flies has three attacks which he can perform while he floats around the room slowly.

  • While moving around the room, he will cough up attack flies that will orbit around him, blocking shots.
  • Occasionally, he will cough up a moving attack fly that will move toward Isaac
  • His third attack consists of him coughing which will spread the flies orbiting him outwards, freeing them to move towards Isaac.
  • Upon death, he will explode into more flies, and all orbiting flies will be free to move towards Isaac.

Champion Versions[edit | edit source]

  • Green Duke of Flies spawns Moters instead of Large Attack Flies and shoots blood shots in eight directions when coughing.
  • Orange Duke of Flies is larger than normal and spawns Suckers instead of Large Attack Flies.

Trivia[edit | edit source]

  • The stitching under the Duke of Flies' mouth is an autopsy stitch, an incision in the shape of a Y. The incision allows passage to the cadaver's insides; when the autopsy is complete, it is stitched back up.


Bosses Boss Monstro.png
Basement MonstroGeminiDingleLarry Jr.The Duke of FliesGurglingsFistulaStevenFamine
Cellar Gurdy Jr.PinWidowBlighted OvumThe HauntDingleLarry Jr.The Duke of FliesGurglingsFistulaFamine
The Caves ChubGurdyGurdy Jr.Mega MawMega FattyDark OneC.H.A.D.Pestilence
Catacombs The HollowThe HuskPeepPolycephalusThe Carrion QueenThe WretchedDark OnePestilence
The Depths The CageMonstro IIThe AdversaryThe GateLokiGishWarMom
Necropolis The BloatThe Carrion QueenMask of InfamyThe WretchedThe GateLokiWarMom
The Womb Mr. FredScolexTeratomaMama GurdyThe BloatLokiiDeathConquestMom's HeartIt Lives
Utero BlastocystDaddy Long LegsTeratomaMama GurdyThe BloatLokiiTriachnidDeathConquestMom's HeartIt Lives
Sheol The FallenSatan
Dark Room The LambMega Satan
Cathedral Isaac
The Chest ???Mega Satan
Devil Room Krampus
Angel Room Angel
Levels 1-5/7 The FallenThe Headless Horseman
Community content is available under CC-BY-SA unless otherwise noted.